augw function Search Results


alkal1  (MedChemExpress)
93
MedChemExpress alkal1
( A ) Peak locations across the genome. The numbers indicate the percentages for ChIP-seq distribution. ( B ) Representative ChIP-seq peak located on the AhR direct target gene revealed using a genome browser Integrative Genomics Viewer. ( C ) Homer known motif enrichment result. ( D ) ChIP-PCR analysis of the selected AhR binding sites. RT-PCR was performed with the indicated primer pair using an anti-AhR antibody immunoprecipitated DNA fragment as the template. Normal mouse IgG was used as negative control. ( E ) RT-PCR analysis of <t>ALKAL1</t> mRNA expression. ( F ) Representative combined staining of CD163 (green), MerTK (pink), ALKAL1 (red), and DAPI (blue) in tumor tissues of patients with melanoma. Scale bars, 25 μm (left panel) and 10 μm (all other panels). ( G and H ) Representative photomicrographs of immunohistochemical analysis for ALKAL1 expression in tumor tissues at various clinicopathologic stages (G), of responders and nonresponders of patients with melanoma treated with anti–PD-1 monoclonal antibodies (H). ALKAL1 was stained in brown. Scale bar, 10 μm. ( I ) Kaplan-Meier analysis of OS curves of melanoma patient datasets from TCGA. ( J and K ) Western blot analysis of phosphorylated and total MerTK proteins following simulation with (+) or without (−) IL-4/IL-13. One representative donor of eight is shown. Numbers depict the quantification of Western blot bands relative to β-actin/glyceraldehyde phosphate dehydrogenase (GAPDH). (J) BMDMs were pretreated with 50 nM siALKAL1 combined with 10 μM CH223191 or 200 μM Kyn for 48 hours. (K) MDMs were pretreated with ALKAL1 protein (1 μg/ml) combined with 10 μM CH223191 for 48 hours. Representative of three experiments. Data are means ± SEM, and P values were determined by two-sided Student’s t test [(D) and (H)], one-way ANOVA [(E) and (G)], or by two-sided Wald test in a Cox-PH regression (I). * P < 0.05, ** P < 0.01, and *** P < 0.001. Chr, chromosome.
Alkal1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aug cc pvdz r moxy  (Fortiori Design LLC)
99
Fortiori Design LLC aug cc pvdz r moxy
( A ) Peak locations across the genome. The numbers indicate the percentages for ChIP-seq distribution. ( B ) Representative ChIP-seq peak located on the AhR direct target gene revealed using a genome browser Integrative Genomics Viewer. ( C ) Homer known motif enrichment result. ( D ) ChIP-PCR analysis of the selected AhR binding sites. RT-PCR was performed with the indicated primer pair using an anti-AhR antibody immunoprecipitated DNA fragment as the template. Normal mouse IgG was used as negative control. ( E ) RT-PCR analysis of <t>ALKAL1</t> mRNA expression. ( F ) Representative combined staining of CD163 (green), MerTK (pink), ALKAL1 (red), and DAPI (blue) in tumor tissues of patients with melanoma. Scale bars, 25 μm (left panel) and 10 μm (all other panels). ( G and H ) Representative photomicrographs of immunohistochemical analysis for ALKAL1 expression in tumor tissues at various clinicopathologic stages (G), of responders and nonresponders of patients with melanoma treated with anti–PD-1 monoclonal antibodies (H). ALKAL1 was stained in brown. Scale bar, 10 μm. ( I ) Kaplan-Meier analysis of OS curves of melanoma patient datasets from TCGA. ( J and K ) Western blot analysis of phosphorylated and total MerTK proteins following simulation with (+) or without (−) IL-4/IL-13. One representative donor of eight is shown. Numbers depict the quantification of Western blot bands relative to β-actin/glyceraldehyde phosphate dehydrogenase (GAPDH). (J) BMDMs were pretreated with 50 nM siALKAL1 combined with 10 μM CH223191 or 200 μM Kyn for 48 hours. (K) MDMs were pretreated with ALKAL1 protein (1 μg/ml) combined with 10 μM CH223191 for 48 hours. Representative of three experiments. Data are means ± SEM, and P values were determined by two-sided Student’s t test [(D) and (H)], one-way ANOVA [(E) and (G)], or by two-sided Wald test in a Cox-PH regression (I). * P < 0.05, ** P < 0.01, and *** P < 0.001. Chr, chromosome.
Aug Cc Pvdz R Moxy, supplied by Fortiori Design LLC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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method of producing functional protein domains  (Serono)
90
Serono method of producing functional protein domains
( A ) Peak locations across the genome. The numbers indicate the percentages for ChIP-seq distribution. ( B ) Representative ChIP-seq peak located on the AhR direct target gene revealed using a genome browser Integrative Genomics Viewer. ( C ) Homer known motif enrichment result. ( D ) ChIP-PCR analysis of the selected AhR binding sites. RT-PCR was performed with the indicated primer pair using an anti-AhR antibody immunoprecipitated DNA fragment as the template. Normal mouse IgG was used as negative control. ( E ) RT-PCR analysis of <t>ALKAL1</t> mRNA expression. ( F ) Representative combined staining of CD163 (green), MerTK (pink), ALKAL1 (red), and DAPI (blue) in tumor tissues of patients with melanoma. Scale bars, 25 μm (left panel) and 10 μm (all other panels). ( G and H ) Representative photomicrographs of immunohistochemical analysis for ALKAL1 expression in tumor tissues at various clinicopathologic stages (G), of responders and nonresponders of patients with melanoma treated with anti–PD-1 monoclonal antibodies (H). ALKAL1 was stained in brown. Scale bar, 10 μm. ( I ) Kaplan-Meier analysis of OS curves of melanoma patient datasets from TCGA. ( J and K ) Western blot analysis of phosphorylated and total MerTK proteins following simulation with (+) or without (−) IL-4/IL-13. One representative donor of eight is shown. Numbers depict the quantification of Western blot bands relative to β-actin/glyceraldehyde phosphate dehydrogenase (GAPDH). (J) BMDMs were pretreated with 50 nM siALKAL1 combined with 10 μM CH223191 or 200 μM Kyn for 48 hours. (K) MDMs were pretreated with ALKAL1 protein (1 μg/ml) combined with 10 μM CH223191 for 48 hours. Representative of three experiments. Data are means ± SEM, and P values were determined by two-sided Student’s t test [(D) and (H)], one-way ANOVA [(E) and (G)], or by two-sided Wald test in a Cox-PH regression (I). * P < 0.05, ** P < 0.01, and *** P < 0.001. Chr, chromosome.
Method Of Producing Functional Protein Domains, supplied by Serono, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ipa version 9.0 software  (Ingenuity Systems)
90
Ingenuity Systems ipa version 9.0 software
( A ) Peak locations across the genome. The numbers indicate the percentages for ChIP-seq distribution. ( B ) Representative ChIP-seq peak located on the AhR direct target gene revealed using a genome browser Integrative Genomics Viewer. ( C ) Homer known motif enrichment result. ( D ) ChIP-PCR analysis of the selected AhR binding sites. RT-PCR was performed with the indicated primer pair using an anti-AhR antibody immunoprecipitated DNA fragment as the template. Normal mouse IgG was used as negative control. ( E ) RT-PCR analysis of <t>ALKAL1</t> mRNA expression. ( F ) Representative combined staining of CD163 (green), MerTK (pink), ALKAL1 (red), and DAPI (blue) in tumor tissues of patients with melanoma. Scale bars, 25 μm (left panel) and 10 μm (all other panels). ( G and H ) Representative photomicrographs of immunohistochemical analysis for ALKAL1 expression in tumor tissues at various clinicopathologic stages (G), of responders and nonresponders of patients with melanoma treated with anti–PD-1 monoclonal antibodies (H). ALKAL1 was stained in brown. Scale bar, 10 μm. ( I ) Kaplan-Meier analysis of OS curves of melanoma patient datasets from TCGA. ( J and K ) Western blot analysis of phosphorylated and total MerTK proteins following simulation with (+) or without (−) IL-4/IL-13. One representative donor of eight is shown. Numbers depict the quantification of Western blot bands relative to β-actin/glyceraldehyde phosphate dehydrogenase (GAPDH). (J) BMDMs were pretreated with 50 nM siALKAL1 combined with 10 μM CH223191 or 200 μM Kyn for 48 hours. (K) MDMs were pretreated with ALKAL1 protein (1 μg/ml) combined with 10 μM CH223191 for 48 hours. Representative of three experiments. Data are means ± SEM, and P values were determined by two-sided Student’s t test [(D) and (H)], one-way ANOVA [(E) and (G)], or by two-sided Wald test in a Cox-PH regression (I). * P < 0.05, ** P < 0.01, and *** P < 0.001. Chr, chromosome.
Ipa Version 9.0 Software, supplied by Ingenuity Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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optical recording material  (Takeda)
90
Takeda optical recording material
( A ) Peak locations across the genome. The numbers indicate the percentages for ChIP-seq distribution. ( B ) Representative ChIP-seq peak located on the AhR direct target gene revealed using a genome browser Integrative Genomics Viewer. ( C ) Homer known motif enrichment result. ( D ) ChIP-PCR analysis of the selected AhR binding sites. RT-PCR was performed with the indicated primer pair using an anti-AhR antibody immunoprecipitated DNA fragment as the template. Normal mouse IgG was used as negative control. ( E ) RT-PCR analysis of <t>ALKAL1</t> mRNA expression. ( F ) Representative combined staining of CD163 (green), MerTK (pink), ALKAL1 (red), and DAPI (blue) in tumor tissues of patients with melanoma. Scale bars, 25 μm (left panel) and 10 μm (all other panels). ( G and H ) Representative photomicrographs of immunohistochemical analysis for ALKAL1 expression in tumor tissues at various clinicopathologic stages (G), of responders and nonresponders of patients with melanoma treated with anti–PD-1 monoclonal antibodies (H). ALKAL1 was stained in brown. Scale bar, 10 μm. ( I ) Kaplan-Meier analysis of OS curves of melanoma patient datasets from TCGA. ( J and K ) Western blot analysis of phosphorylated and total MerTK proteins following simulation with (+) or without (−) IL-4/IL-13. One representative donor of eight is shown. Numbers depict the quantification of Western blot bands relative to β-actin/glyceraldehyde phosphate dehydrogenase (GAPDH). (J) BMDMs were pretreated with 50 nM siALKAL1 combined with 10 μM CH223191 or 200 μM Kyn for 48 hours. (K) MDMs were pretreated with ALKAL1 protein (1 μg/ml) combined with 10 μM CH223191 for 48 hours. Representative of three experiments. Data are means ± SEM, and P values were determined by two-sided Student’s t test [(D) and (H)], one-way ANOVA [(E) and (G)], or by two-sided Wald test in a Cox-PH regression (I). * P < 0.05, ** P < 0.01, and *** P < 0.001. Chr, chromosome.
Optical Recording Material, supplied by Takeda, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nyha functional classification  (Boehringer Ingelheim)
90
Boehringer Ingelheim nyha functional classification
( A ) Peak locations across the genome. The numbers indicate the percentages for ChIP-seq distribution. ( B ) Representative ChIP-seq peak located on the AhR direct target gene revealed using a genome browser Integrative Genomics Viewer. ( C ) Homer known motif enrichment result. ( D ) ChIP-PCR analysis of the selected AhR binding sites. RT-PCR was performed with the indicated primer pair using an anti-AhR antibody immunoprecipitated DNA fragment as the template. Normal mouse IgG was used as negative control. ( E ) RT-PCR analysis of <t>ALKAL1</t> mRNA expression. ( F ) Representative combined staining of CD163 (green), MerTK (pink), ALKAL1 (red), and DAPI (blue) in tumor tissues of patients with melanoma. Scale bars, 25 μm (left panel) and 10 μm (all other panels). ( G and H ) Representative photomicrographs of immunohistochemical analysis for ALKAL1 expression in tumor tissues at various clinicopathologic stages (G), of responders and nonresponders of patients with melanoma treated with anti–PD-1 monoclonal antibodies (H). ALKAL1 was stained in brown. Scale bar, 10 μm. ( I ) Kaplan-Meier analysis of OS curves of melanoma patient datasets from TCGA. ( J and K ) Western blot analysis of phosphorylated and total MerTK proteins following simulation with (+) or without (−) IL-4/IL-13. One representative donor of eight is shown. Numbers depict the quantification of Western blot bands relative to β-actin/glyceraldehyde phosphate dehydrogenase (GAPDH). (J) BMDMs were pretreated with 50 nM siALKAL1 combined with 10 μM CH223191 or 200 μM Kyn for 48 hours. (K) MDMs were pretreated with ALKAL1 protein (1 μg/ml) combined with 10 μM CH223191 for 48 hours. Representative of three experiments. Data are means ± SEM, and P values were determined by two-sided Student’s t test [(D) and (H)], one-way ANOVA [(E) and (G)], or by two-sided Wald test in a Cox-PH regression (I). * P < 0.05, ** P < 0.01, and *** P < 0.001. Chr, chromosome.
Nyha Functional Classification, supplied by Boehringer Ingelheim, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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virtex4  (Xilinx Inc)
90
Xilinx Inc virtex4
( A ) Peak locations across the genome. The numbers indicate the percentages for ChIP-seq distribution. ( B ) Representative ChIP-seq peak located on the AhR direct target gene revealed using a genome browser Integrative Genomics Viewer. ( C ) Homer known motif enrichment result. ( D ) ChIP-PCR analysis of the selected AhR binding sites. RT-PCR was performed with the indicated primer pair using an anti-AhR antibody immunoprecipitated DNA fragment as the template. Normal mouse IgG was used as negative control. ( E ) RT-PCR analysis of <t>ALKAL1</t> mRNA expression. ( F ) Representative combined staining of CD163 (green), MerTK (pink), ALKAL1 (red), and DAPI (blue) in tumor tissues of patients with melanoma. Scale bars, 25 μm (left panel) and 10 μm (all other panels). ( G and H ) Representative photomicrographs of immunohistochemical analysis for ALKAL1 expression in tumor tissues at various clinicopathologic stages (G), of responders and nonresponders of patients with melanoma treated with anti–PD-1 monoclonal antibodies (H). ALKAL1 was stained in brown. Scale bar, 10 μm. ( I ) Kaplan-Meier analysis of OS curves of melanoma patient datasets from TCGA. ( J and K ) Western blot analysis of phosphorylated and total MerTK proteins following simulation with (+) or without (−) IL-4/IL-13. One representative donor of eight is shown. Numbers depict the quantification of Western blot bands relative to β-actin/glyceraldehyde phosphate dehydrogenase (GAPDH). (J) BMDMs were pretreated with 50 nM siALKAL1 combined with 10 μM CH223191 or 200 μM Kyn for 48 hours. (K) MDMs were pretreated with ALKAL1 protein (1 μg/ml) combined with 10 μM CH223191 for 48 hours. Representative of three experiments. Data are means ± SEM, and P values were determined by two-sided Student’s t test [(D) and (H)], one-way ANOVA [(E) and (G)], or by two-sided Wald test in a Cox-PH regression (I). * P < 0.05, ** P < 0.01, and *** P < 0.001. Chr, chromosome.
Virtex4, supplied by Xilinx Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirna duplexes against human hp sr302217a  (OriGene)
90
OriGene sirna duplexes against human hp sr302217a
( A ) Peak locations across the genome. The numbers indicate the percentages for ChIP-seq distribution. ( B ) Representative ChIP-seq peak located on the AhR direct target gene revealed using a genome browser Integrative Genomics Viewer. ( C ) Homer known motif enrichment result. ( D ) ChIP-PCR analysis of the selected AhR binding sites. RT-PCR was performed with the indicated primer pair using an anti-AhR antibody immunoprecipitated DNA fragment as the template. Normal mouse IgG was used as negative control. ( E ) RT-PCR analysis of <t>ALKAL1</t> mRNA expression. ( F ) Representative combined staining of CD163 (green), MerTK (pink), ALKAL1 (red), and DAPI (blue) in tumor tissues of patients with melanoma. Scale bars, 25 μm (left panel) and 10 μm (all other panels). ( G and H ) Representative photomicrographs of immunohistochemical analysis for ALKAL1 expression in tumor tissues at various clinicopathologic stages (G), of responders and nonresponders of patients with melanoma treated with anti–PD-1 monoclonal antibodies (H). ALKAL1 was stained in brown. Scale bar, 10 μm. ( I ) Kaplan-Meier analysis of OS curves of melanoma patient datasets from TCGA. ( J and K ) Western blot analysis of phosphorylated and total MerTK proteins following simulation with (+) or without (−) IL-4/IL-13. One representative donor of eight is shown. Numbers depict the quantification of Western blot bands relative to β-actin/glyceraldehyde phosphate dehydrogenase (GAPDH). (J) BMDMs were pretreated with 50 nM siALKAL1 combined with 10 μM CH223191 or 200 μM Kyn for 48 hours. (K) MDMs were pretreated with ALKAL1 protein (1 μg/ml) combined with 10 μM CH223191 for 48 hours. Representative of three experiments. Data are means ± SEM, and P values were determined by two-sided Student’s t test [(D) and (H)], one-way ANOVA [(E) and (G)], or by two-sided Wald test in a Cox-PH regression (I). * P < 0.05, ** P < 0.01, and *** P < 0.001. Chr, chromosome.
Sirna Duplexes Against Human Hp Sr302217a, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cc2 computations  (TURBOMOLE GmbH)
90
TURBOMOLE GmbH cc2 computations
( A ) Peak locations across the genome. The numbers indicate the percentages for ChIP-seq distribution. ( B ) Representative ChIP-seq peak located on the AhR direct target gene revealed using a genome browser Integrative Genomics Viewer. ( C ) Homer known motif enrichment result. ( D ) ChIP-PCR analysis of the selected AhR binding sites. RT-PCR was performed with the indicated primer pair using an anti-AhR antibody immunoprecipitated DNA fragment as the template. Normal mouse IgG was used as negative control. ( E ) RT-PCR analysis of <t>ALKAL1</t> mRNA expression. ( F ) Representative combined staining of CD163 (green), MerTK (pink), ALKAL1 (red), and DAPI (blue) in tumor tissues of patients with melanoma. Scale bars, 25 μm (left panel) and 10 μm (all other panels). ( G and H ) Representative photomicrographs of immunohistochemical analysis for ALKAL1 expression in tumor tissues at various clinicopathologic stages (G), of responders and nonresponders of patients with melanoma treated with anti–PD-1 monoclonal antibodies (H). ALKAL1 was stained in brown. Scale bar, 10 μm. ( I ) Kaplan-Meier analysis of OS curves of melanoma patient datasets from TCGA. ( J and K ) Western blot analysis of phosphorylated and total MerTK proteins following simulation with (+) or without (−) IL-4/IL-13. One representative donor of eight is shown. Numbers depict the quantification of Western blot bands relative to β-actin/glyceraldehyde phosphate dehydrogenase (GAPDH). (J) BMDMs were pretreated with 50 nM siALKAL1 combined with 10 μM CH223191 or 200 μM Kyn for 48 hours. (K) MDMs were pretreated with ALKAL1 protein (1 μg/ml) combined with 10 μM CH223191 for 48 hours. Representative of three experiments. Data are means ± SEM, and P values were determined by two-sided Student’s t test [(D) and (H)], one-way ANOVA [(E) and (G)], or by two-sided Wald test in a Cox-PH regression (I). * P < 0.05, ** P < 0.01, and *** P < 0.001. Chr, chromosome.
Cc2 Computations, supplied by TURBOMOLE GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peptide-functionalized quantum dot biosensors  (Quantum Dot Inc)
90
Quantum Dot Inc peptide-functionalized quantum dot biosensors
( A ) Peak locations across the genome. The numbers indicate the percentages for ChIP-seq distribution. ( B ) Representative ChIP-seq peak located on the AhR direct target gene revealed using a genome browser Integrative Genomics Viewer. ( C ) Homer known motif enrichment result. ( D ) ChIP-PCR analysis of the selected AhR binding sites. RT-PCR was performed with the indicated primer pair using an anti-AhR antibody immunoprecipitated DNA fragment as the template. Normal mouse IgG was used as negative control. ( E ) RT-PCR analysis of <t>ALKAL1</t> mRNA expression. ( F ) Representative combined staining of CD163 (green), MerTK (pink), ALKAL1 (red), and DAPI (blue) in tumor tissues of patients with melanoma. Scale bars, 25 μm (left panel) and 10 μm (all other panels). ( G and H ) Representative photomicrographs of immunohistochemical analysis for ALKAL1 expression in tumor tissues at various clinicopathologic stages (G), of responders and nonresponders of patients with melanoma treated with anti–PD-1 monoclonal antibodies (H). ALKAL1 was stained in brown. Scale bar, 10 μm. ( I ) Kaplan-Meier analysis of OS curves of melanoma patient datasets from TCGA. ( J and K ) Western blot analysis of phosphorylated and total MerTK proteins following simulation with (+) or without (−) IL-4/IL-13. One representative donor of eight is shown. Numbers depict the quantification of Western blot bands relative to β-actin/glyceraldehyde phosphate dehydrogenase (GAPDH). (J) BMDMs were pretreated with 50 nM siALKAL1 combined with 10 μM CH223191 or 200 μM Kyn for 48 hours. (K) MDMs were pretreated with ALKAL1 protein (1 μg/ml) combined with 10 μM CH223191 for 48 hours. Representative of three experiments. Data are means ± SEM, and P values were determined by two-sided Student’s t test [(D) and (H)], one-way ANOVA [(E) and (G)], or by two-sided Wald test in a Cox-PH regression (I). * P < 0.05, ** P < 0.01, and *** P < 0.001. Chr, chromosome.
Peptide Functionalized Quantum Dot Biosensors, supplied by Quantum Dot Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tip aug 2016 3698 3711 transfer functions robust w  (Thermo Fisher)
95
Thermo Fisher tip aug 2016 3698 3711 transfer functions robust w
( A ) Peak locations across the genome. The numbers indicate the percentages for ChIP-seq distribution. ( B ) Representative ChIP-seq peak located on the AhR direct target gene revealed using a genome browser Integrative Genomics Viewer. ( C ) Homer known motif enrichment result. ( D ) ChIP-PCR analysis of the selected AhR binding sites. RT-PCR was performed with the indicated primer pair using an anti-AhR antibody immunoprecipitated DNA fragment as the template. Normal mouse IgG was used as negative control. ( E ) RT-PCR analysis of <t>ALKAL1</t> mRNA expression. ( F ) Representative combined staining of CD163 (green), MerTK (pink), ALKAL1 (red), and DAPI (blue) in tumor tissues of patients with melanoma. Scale bars, 25 μm (left panel) and 10 μm (all other panels). ( G and H ) Representative photomicrographs of immunohistochemical analysis for ALKAL1 expression in tumor tissues at various clinicopathologic stages (G), of responders and nonresponders of patients with melanoma treated with anti–PD-1 monoclonal antibodies (H). ALKAL1 was stained in brown. Scale bar, 10 μm. ( I ) Kaplan-Meier analysis of OS curves of melanoma patient datasets from TCGA. ( J and K ) Western blot analysis of phosphorylated and total MerTK proteins following simulation with (+) or without (−) IL-4/IL-13. One representative donor of eight is shown. Numbers depict the quantification of Western blot bands relative to β-actin/glyceraldehyde phosphate dehydrogenase (GAPDH). (J) BMDMs were pretreated with 50 nM siALKAL1 combined with 10 μM CH223191 or 200 μM Kyn for 48 hours. (K) MDMs were pretreated with ALKAL1 protein (1 μg/ml) combined with 10 μM CH223191 for 48 hours. Representative of three experiments. Data are means ± SEM, and P values were determined by two-sided Student’s t test [(D) and (H)], one-way ANOVA [(E) and (G)], or by two-sided Wald test in a Cox-PH regression (I). * P < 0.05, ** P < 0.01, and *** P < 0.001. Chr, chromosome.
Tip Aug 2016 3698 3711 Transfer Functions Robust W, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Peak locations across the genome. The numbers indicate the percentages for ChIP-seq distribution. ( B ) Representative ChIP-seq peak located on the AhR direct target gene revealed using a genome browser Integrative Genomics Viewer. ( C ) Homer known motif enrichment result. ( D ) ChIP-PCR analysis of the selected AhR binding sites. RT-PCR was performed with the indicated primer pair using an anti-AhR antibody immunoprecipitated DNA fragment as the template. Normal mouse IgG was used as negative control. ( E ) RT-PCR analysis of ALKAL1 mRNA expression. ( F ) Representative combined staining of CD163 (green), MerTK (pink), ALKAL1 (red), and DAPI (blue) in tumor tissues of patients with melanoma. Scale bars, 25 μm (left panel) and 10 μm (all other panels). ( G and H ) Representative photomicrographs of immunohistochemical analysis for ALKAL1 expression in tumor tissues at various clinicopathologic stages (G), of responders and nonresponders of patients with melanoma treated with anti–PD-1 monoclonal antibodies (H). ALKAL1 was stained in brown. Scale bar, 10 μm. ( I ) Kaplan-Meier analysis of OS curves of melanoma patient datasets from TCGA. ( J and K ) Western blot analysis of phosphorylated and total MerTK proteins following simulation with (+) or without (−) IL-4/IL-13. One representative donor of eight is shown. Numbers depict the quantification of Western blot bands relative to β-actin/glyceraldehyde phosphate dehydrogenase (GAPDH). (J) BMDMs were pretreated with 50 nM siALKAL1 combined with 10 μM CH223191 or 200 μM Kyn for 48 hours. (K) MDMs were pretreated with ALKAL1 protein (1 μg/ml) combined with 10 μM CH223191 for 48 hours. Representative of three experiments. Data are means ± SEM, and P values were determined by two-sided Student’s t test [(D) and (H)], one-way ANOVA [(E) and (G)], or by two-sided Wald test in a Cox-PH regression (I). * P < 0.05, ** P < 0.01, and *** P < 0.001. Chr, chromosome.

Journal: Science Advances

Article Title: MerTK + macrophages promote melanoma progression and immunotherapy resistance through AhR-ALKAL1 activation

doi: 10.1126/sciadv.ado8366

Figure Lengend Snippet: ( A ) Peak locations across the genome. The numbers indicate the percentages for ChIP-seq distribution. ( B ) Representative ChIP-seq peak located on the AhR direct target gene revealed using a genome browser Integrative Genomics Viewer. ( C ) Homer known motif enrichment result. ( D ) ChIP-PCR analysis of the selected AhR binding sites. RT-PCR was performed with the indicated primer pair using an anti-AhR antibody immunoprecipitated DNA fragment as the template. Normal mouse IgG was used as negative control. ( E ) RT-PCR analysis of ALKAL1 mRNA expression. ( F ) Representative combined staining of CD163 (green), MerTK (pink), ALKAL1 (red), and DAPI (blue) in tumor tissues of patients with melanoma. Scale bars, 25 μm (left panel) and 10 μm (all other panels). ( G and H ) Representative photomicrographs of immunohistochemical analysis for ALKAL1 expression in tumor tissues at various clinicopathologic stages (G), of responders and nonresponders of patients with melanoma treated with anti–PD-1 monoclonal antibodies (H). ALKAL1 was stained in brown. Scale bar, 10 μm. ( I ) Kaplan-Meier analysis of OS curves of melanoma patient datasets from TCGA. ( J and K ) Western blot analysis of phosphorylated and total MerTK proteins following simulation with (+) or without (−) IL-4/IL-13. One representative donor of eight is shown. Numbers depict the quantification of Western blot bands relative to β-actin/glyceraldehyde phosphate dehydrogenase (GAPDH). (J) BMDMs were pretreated with 50 nM siALKAL1 combined with 10 μM CH223191 or 200 μM Kyn for 48 hours. (K) MDMs were pretreated with ALKAL1 protein (1 μg/ml) combined with 10 μM CH223191 for 48 hours. Representative of three experiments. Data are means ± SEM, and P values were determined by two-sided Student’s t test [(D) and (H)], one-way ANOVA [(E) and (G)], or by two-sided Wald test in a Cox-PH regression (I). * P < 0.05, ** P < 0.01, and *** P < 0.001. Chr, chromosome.

Article Snippet: ALKAL1 (1 μg/ml; MCE) combined with 10 μM CH223191 (Sigma-Aldrich) were added to the MDM polarization culture.

Techniques: ChIP-sequencing, Binding Assay, Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation, Negative Control, Expressing, Staining, Immunohistochemical staining, Bioprocessing, Western Blot

( A ) Representative combined staining of F4/80 (green) and AhR (red) in BMDMs of the indicated genotype. DAPI (blue) was the nuclear stain. Scale bar, 10 μm. ( B ) RT-PCR analysis of ALKAL1 and MerTK mRNA expression in BMDMs of the indicated genotype. ( C ) Western blot analysis of phosphorylated and total MerTK proteins with (+) or without (−) IL-4 polarization in BMDMs of the indicated genotype for 24 hours. One representative donor of four is shown. Numbers depict the quantification of Western blot bands relative to β-actin. ( D to K ) Studying the effect of MerTK + Mac in a Lyz cre/+ Ahr fl/fl B16-F10 syngeneic mouse tumor model. (D) Adoptive transfer method. (E) Tumor growth. (F) Subcutaneous B16-F10 tumors were surgically removed and presented. Scale bar, 1 cm. (G) Tumor weight. (H) Flow cytometry analysis of the percentages of PD-L1 + cells in MerTK + Mac. (I) Flow cytometry analysis of the percentages of CD8 + T cells in CD3 + T cells. [(J) and (K)] Flow cytometry analysis of the percentages of cells expressing IFN-γ and TNF-α (J) and PD-1 (K) in CD4 + and CD8 + T cells. ( L ) Experimental design. ( M ) Flow cytometry analysis of the percentage of live tumor cells (gated as hCD3 − hCD114 − annexin V − PI − cells). ( N and O ) Flow cytometry analysis of the percentage of CD69 (N), IFN-γ and TNF-α (O) in CD8 + T cells. Representative of three experiments. Data are means ± SEM, and P values were determined by two-way ANOVA [(B) and (G) to (K)] or by one-way ANOVA [(E) and (M) to (O)]. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Journal: Science Advances

Article Title: MerTK + macrophages promote melanoma progression and immunotherapy resistance through AhR-ALKAL1 activation

doi: 10.1126/sciadv.ado8366

Figure Lengend Snippet: ( A ) Representative combined staining of F4/80 (green) and AhR (red) in BMDMs of the indicated genotype. DAPI (blue) was the nuclear stain. Scale bar, 10 μm. ( B ) RT-PCR analysis of ALKAL1 and MerTK mRNA expression in BMDMs of the indicated genotype. ( C ) Western blot analysis of phosphorylated and total MerTK proteins with (+) or without (−) IL-4 polarization in BMDMs of the indicated genotype for 24 hours. One representative donor of four is shown. Numbers depict the quantification of Western blot bands relative to β-actin. ( D to K ) Studying the effect of MerTK + Mac in a Lyz cre/+ Ahr fl/fl B16-F10 syngeneic mouse tumor model. (D) Adoptive transfer method. (E) Tumor growth. (F) Subcutaneous B16-F10 tumors were surgically removed and presented. Scale bar, 1 cm. (G) Tumor weight. (H) Flow cytometry analysis of the percentages of PD-L1 + cells in MerTK + Mac. (I) Flow cytometry analysis of the percentages of CD8 + T cells in CD3 + T cells. [(J) and (K)] Flow cytometry analysis of the percentages of cells expressing IFN-γ and TNF-α (J) and PD-1 (K) in CD4 + and CD8 + T cells. ( L ) Experimental design. ( M ) Flow cytometry analysis of the percentage of live tumor cells (gated as hCD3 − hCD114 − annexin V − PI − cells). ( N and O ) Flow cytometry analysis of the percentage of CD69 (N), IFN-γ and TNF-α (O) in CD8 + T cells. Representative of three experiments. Data are means ± SEM, and P values were determined by two-way ANOVA [(B) and (G) to (K)] or by one-way ANOVA [(E) and (M) to (O)]. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: ALKAL1 (1 μg/ml; MCE) combined with 10 μM CH223191 (Sigma-Aldrich) were added to the MDM polarization culture.

Techniques: Staining, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Adoptive Transfer Assay, Flow Cytometry

Reagent information. N/A, not applicable.

Journal: Science Advances

Article Title: MerTK + macrophages promote melanoma progression and immunotherapy resistance through AhR-ALKAL1 activation

doi: 10.1126/sciadv.ado8366

Figure Lengend Snippet: Reagent information. N/A, not applicable.

Article Snippet: ALKAL1 (1 μg/ml; MCE) combined with 10 μM CH223191 (Sigma-Aldrich) were added to the MDM polarization culture.

Techniques: Recombinant, Purification, Functional Assay, Control, Protease Inhibitor, Staining, Liposomes, Enzyme-linked Immunosorbent Assay, Selection, Chromatin Immunoprecipitation, Magnetic Beads, Bicinchoninic Acid Protein Assay, cDNA Synthesis, Transfection